In a parallel process, SmartZyme’s protein engineers conduct
in-depth literature review and analysis for the development, adaptation and
invention of assays to select the most promising protein mutants.
Structural biologists utilize experimental and in-silico approaches for the generation
of protein models that further support protein engineering methodologies and
focus on the construction and assembly of mutant libraries.
Once these libraries are generated, the vast number of protein
mutants are screened with basic and advanced assays, several of which highly
mimic real life conditions.
The company invests significant resources in continuous research
toward the development of accurate and reliable screening assays.
As part of the assembly of libraries, the team screens for
multiple protein expression systems (microbial or mammalian) and thoroughly evaluates
them. This evaluation leads to identifying the best yield and production
capabilities, which is a key initiative in the scale up process.